Difference between revisions of "Part:BBa K322999:Experience"

Latest revision as of 01:25, 28 October 2010

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Applications of BBa_K322999

The response of two clones of LovTAP under blue light (controls were not included in this experiment). There is unfortunately no clear difference in response (measured by fluorescence/optical density) over time. Expected results would show higher fluorescence in the dark than in the light.

The above shows the same experiment performed on a TrpR mutant - to see if there is any difference in LovTAP activation under blue light illumination. Again, fluorescence should be higher in the dark state - due to RFP reporter being suppressed by blue light - of LovTAP activation. Again, however, results are inconclusive.

User Reviews

UNIQ850df1998671885e-partinfo-00000000-QINU UNIQ850df1998671885e-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K322999===
+https://static.igem.org/mediawiki/2010/9/91/8octLovtap.jpg
+The response of two clones of LovTAP under blue light (controls were not included in this experiment). There is unfortunately no clear difference in response (measured by fluorescence/optical density) over time. Expected results would show higher fluorescence in the dark than in the light.
+https://static.igem.org/mediawiki/2010/3/38/8octTRPR.jpg
+The above shows the same experiment performed on a TrpR mutant - to see if there is any difference in LovTAP activation under blue light illumination. Again, fluorescence should be higher in the dark state - due to RFP reporter being suppressed by blue light - of LovTAP activation. Again, however, results are inconclusive.
 ===User Reviews===