Difference between revisions of "Part:BBa K318516:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The ramA gene was PCR Amplified from ''Salmonella enterica'' strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts). It does have a PstI cut site within the insert. | |
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===Source=== | ===Source=== |
Revision as of 01:16, 28 October 2010
RamA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 116
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 116
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 116
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 116
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ramA gene was PCR Amplified from Salmonella enterica strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts). It does have a PstI cut site within the insert.
Source
The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.