Difference between revisions of "Part:BBa K322246"
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<partinfo>BBa_K322246 short</partinfo> | <partinfo>BBa_K322246 short</partinfo> | ||
| − | Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter. | + | Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter than BBa_K322211. |
In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light | In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light | ||
Latest revision as of 01:09, 28 October 2010
Red emitting firefly luciferase mutant (S284T)
Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter than BBa_K322211.
In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light
This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.
Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 808