Difference between revisions of "Part:BBa K322247"

(New page: This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red. Note that firefly luciferase is temperature sensitive...)
 
 
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This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red.  
 
This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red.  
 
Note that firefly luciferase is temperature sensitive, and cells grown at 30°C are considerably brighter than cells grown at 37°C.
 
Note that firefly luciferase is temperature sensitive, and cells grown at 30°C are considerably brighter than cells grown at 37°C.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K322246 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K322246 parameters</partinfo>
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Latest revision as of 01:00, 28 October 2010

This part is made of the lac promoter followed by S284T firefly luciferase mutant, which shifts its emission spectrum to make it red. Note that firefly luciferase is temperature sensitive, and cells grown at 30°C are considerably brighter than cells grown at 37°C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808