Difference between revisions of "Part:BBa I10001"

 
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Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)
 
Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)
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===Usage and Biology===
 
===Usage and Biology===
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Chris Anderson & colleagues have demonstrated that the display of invasin on E. coli allows invasin-mediated internalization by mammalian cells; such update does not require additional known adhesion molecules.  Their work was done in E. coli MC1061, a fim+ strain, and CAMC600, a fim- strain (Andereson et al., 2005 PMID: 16330045).  Inv+ E. coli can invade a broad range of tumor cells (epithelial, hepatocarcinoma, and osteosarcoma).
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Chris Anderson & colleagues have demonstrated that the display of invasin on E. coli allows invasin-mediated internalization by mammalian cells; such update does not require additional known adhesion molecules.  Their work was done in E. coli MC1061, a fim+ strain, and CAMC600, a fim- strain (Andereson et al., 2005 PMID: 16330045).  Inv+ E. coli can invade a broad range of tumor cells (epithelial, hepatocarcinoma, and osteosarcoma).
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_I10001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_I10001 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_I10001 parameters</partinfo>
 
<partinfo>BBa_I10001 parameters</partinfo>
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Revision as of 01:22, 5 June 2006


Invasin

Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)


Usage and Biology

Chris Anderson & colleagues have demonstrated that the display of invasin on E. coli allows invasin-mediated internalization by mammalian cells; such update does not require additional known adhesion molecules. Their work was done in E. coli MC1061, a fim+ strain, and CAMC600, a fim- strain (Andereson et al., 2005 PMID: 16330045). Inv+ E. coli can invade a broad range of tumor cells (epithelial, hepatocarcinoma, and osteosarcoma).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 840
    Illegal AgeI site found at 349
    Illegal AgeI site found at 673
    Illegal AgeI site found at 808
    Illegal AgeI site found at 886
    Illegal AgeI site found at 2395
    Illegal AgeI site found at 2899
  • 1000
    COMPATIBLE WITH RFC[1000]