Difference between revisions of "Part:BBa K398001"
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This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with [https://parts.igem.org/Part:BBa_K398002 Rubredoxin 3], [https://parts.igem.org/Part:BBa_K398003 Rubredoxin 4] and [https://parts.igem.org/Part:BBa_K398004 Rubredoxin Reductase] it forms the minimal component alkane hydroxylase system. For more information see the references below. | This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with [https://parts.igem.org/Part:BBa_K398002 Rubredoxin 3], [https://parts.igem.org/Part:BBa_K398003 Rubredoxin 4] and [https://parts.igem.org/Part:BBa_K398004 Rubredoxin Reductase] it forms the minimal component alkane hydroxylase system. For more information see the references below. | ||
| − | See [https://parts.igem.org/Part:BBa_K398014 | + | See [https://parts.igem.org/Part:BBa_K398014 BBa_K398014] for the characterized protein generator. |
[1] Fujii, T., et al., <i>Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6.</i> Bioscience Biotechnology and Biochemistry, 2004. <b>68</b>(10): p. 2171-2177. | [1] Fujii, T., et al., <i>Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6.</i> Bioscience Biotechnology and Biochemistry, 2004. <b>68</b>(10): p. 2171-2177. | ||
Latest revision as of 00:34, 28 October 2010
Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)
This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with Rubredoxin 3, Rubredoxin 4 and Rubredoxin Reductase it forms the minimal component alkane hydroxylase system. For more information see the references below.
See BBa_K398014 for the characterized protein generator.
[1] Fujii, T., et al., Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6. Bioscience Biotechnology and Biochemistry, 2004. 68(10): p. 2171-2177.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]