Difference between revisions of "Part:BBa K398001"
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This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with [https://parts.igem.org/Part:BBa_K398002 Rubredoxin 3], [https://parts.igem.org/Part:BBa_K398003 Rubredoxin 4] and [https://parts.igem.org/Part:BBa_K398004 Rubredoxin Reductase] it forms the minimal component alkane hydroxylase system. For more information see the references below.
 
This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with [https://parts.igem.org/Part:BBa_K398002 Rubredoxin 3], [https://parts.igem.org/Part:BBa_K398003 Rubredoxin 4] and [https://parts.igem.org/Part:BBa_K398004 Rubredoxin Reductase] it forms the minimal component alkane hydroxylase system. For more information see the references below.
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See [https://parts.igem.org/Part:BBa_K398014 BBa_K398017] for the characterized protein generator.
  
 
[1] Fujii, T., et al., <i>Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6.</i> Bioscience Biotechnology and Biochemistry, 2004. <b>68</b>(10): p. 2171-2177.
 
[1] Fujii, T., et al., <i>Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6.</i> Bioscience Biotechnology and Biochemistry, 2004. <b>68</b>(10): p. 2171-2177.

Revision as of 00:21, 28 October 2010

Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)

This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with Rubredoxin 3, Rubredoxin 4 and Rubredoxin Reductase it forms the minimal component alkane hydroxylase system. For more information see the references below.

See BBa_K398017 for the characterized protein generator.

[1] Fujii, T., et al., Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6. Bioscience Biotechnology and Biochemistry, 2004. 68(10): p. 2171-2177.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]