Difference between revisions of "Part:BBa K342000:Experience"

(→‎Applications of BBa_K342000)
(→‎Applications of BBa_K342000)
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<li> temperature : 28°C  
 
<li> temperature : 28°C  
 
<li> shaking speed : 100 rpm  
 
<li> shaking speed : 100 rpm  
−
<li> osmotic pressure : 0,01 M  
+
<li> osmotic pressure : 0,01 M (sucrose)
 
<br/>
 
<br/>
 
<br/> Out of these data, the expression of this promoter is decreasing quickly until a total lack of expression.
 
<br/> Out of these data, the expression of this promoter is decreasing quickly until a total lack of expression.

Revision as of 00:20, 28 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K342000

This part is a promoter sensible to temperature, shaking speed and osmotic pressure. It is an inductible promoter which best rate of expression is obtained with these conditions:

  • temperature : 28°C
  • shaking speed : 100 rpm
  • osmotic pressure : 0,01 M (sucrose)

    Out of these data, the expression of this promoter is decreasing quickly until a total lack of expression.
    Expression is estimated using a gfp gene reporter gene, by measuring the ratio Fluorescence/ biomass.

    User Reviews

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