Difference between revisions of "Part:BBa K395306:Experience"
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<I>Tokyo Tech iGEM2010</I> | <I>Tokyo Tech iGEM2010</I> | ||
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− | [[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium | + | [[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 houts. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010]] |
In order to characterize P''ompC(C)'' [https://parts.igem.org/Part:BBa_K395301 BBa_395301], P''ompC(CB)'' [https://parts.igem.org/Part:BBa_K395302 BBa395302] and P''ompC(CS1)'' [https://parts.igem.org/Part:BBa_K395303 BBa_395303], each promoter was attached to GFP and its transcriptional activity was measured through the GFP expression. <br><br> | In order to characterize P''ompC(C)'' [https://parts.igem.org/Part:BBa_K395301 BBa_395301], P''ompC(CB)'' [https://parts.igem.org/Part:BBa_K395302 BBa395302] and P''ompC(CS1)'' [https://parts.igem.org/Part:BBa_K395303 BBa_395303], each promoter was attached to GFP and its transcriptional activity was measured through the GFP expression. <br><br> | ||
P''ompC(C)''-GFP[https://parts.igem.org/Part:BBa_K395304 BBa_395304] , P''ompC(CB)''-GFP [https://parts.igem.org/Part:BBa_K395305 BBa_395305], P''ompC(CS1)''-GFP [https://parts.igem.org/Part:BBa_K395306 BBa_395306]and P''ompC(WT)''-GFP[https://parts.igem.org/Part:BBa_K395307 BBa_395307] on pSB3K3 was introduced into E. coli strain MG1655. A strain containing placI<sup>q</sup>-GFP, plasmid (BBa_J54202), a constitutive GFP expressive promoter and promoterless GFP reporter plasmid (BBa_J54103) were used as a positive control and negative control respectively. <br><br> | P''ompC(C)''-GFP[https://parts.igem.org/Part:BBa_K395304 BBa_395304] , P''ompC(CB)''-GFP [https://parts.igem.org/Part:BBa_K395305 BBa_395305], P''ompC(CS1)''-GFP [https://parts.igem.org/Part:BBa_K395306 BBa_395306]and P''ompC(WT)''-GFP[https://parts.igem.org/Part:BBa_K395307 BBa_395307] on pSB3K3 was introduced into E. coli strain MG1655. A strain containing placI<sup>q</sup>-GFP, plasmid (BBa_J54202), a constitutive GFP expressive promoter and promoterless GFP reporter plasmid (BBa_J54103) were used as a positive control and negative control respectively. <br><br> |
Revision as of 00:17, 28 October 2010
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UNIQd9415b7e468879ec-partinfo-00000000-QINU
- Characterization of new series of OmpC propmoters
Tokyo Tech iGEM2010 |
In order to characterize PompC(C) BBa_395301, PompC(CB) BBa395302 and PompC(CS1) BBa_395303, each promoter was attached to GFP and its transcriptional activity was measured through the GFP expression. |
UNIQd9415b7e468879ec-partinfo-00000001-QINU