Difference between revisions of "Part:BBa I13521:Experience"
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===Applications of BBa_I13521===
 
===Applications of BBa_I13521===
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<br>For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.
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<html> <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 591px; height: 313px;" alt="w7" src="https://static.igem.org/mediawiki/2010/3/3a/C1.GIF"></a></div> </html>
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<br> The graph above shows the emission scan for RFP in part BBa_I13521. Since it was the best option in terms of fluorescence yield, 570 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 605 nm.
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<br>Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.
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<br>For the parts BBa_I13521 and BBa_I13522 which are mentioned above, confocal laser scanning microscope images were taken in the host Top Ten.
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<html> <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 350px; height: 350px;" alt="w7" src="https://static.igem.org/mediawiki/2010/1/1a/C4.GIF"></a></div></html>
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Characterization was performed by METU-TURKEY IGEM2010 Team
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:58, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I13521



For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.

w7


The graph above shows the emission scan for RFP in part BBa_I13521. Since it was the best option in terms of fluorescence yield, 570 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 605 nm.
Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.

For the parts BBa_I13521 and BBa_I13522 which are mentioned above, confocal laser scanning microscope images were taken in the host Top Ten.
w7

Characterization was performed by METU-TURKEY IGEM2010 Team

User Reviews

UNIQ5fac8be5cf1c78c8-partinfo-00000002-QINU

•••••

melissali

Very visible to the naked eye (vs. CFP, YFP were not very visible without UV excitation).

UNIQ5fac8be5cf1c78c8-partinfo-00000004-QINU