Difference between revisions of "Part:BBa K361011"
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<partinfo>BBa_K361011 short</partinfo>
 
<partinfo>BBa_K361011 short</partinfo>
  
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This is the protein genertor of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
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This is the protein genertor of Rci site-specific recombinase BBa_K361000, for which a promotor and a ribosome binding site are linked on the left flank and a double bidirectional terminator on the right flank of the recombinase.
  
  

Revision as of 22:49, 27 October 2010

Rci recombinase system

This is the protein genertor of Rci site-specific recombinase BBa_K361000, for which a promotor and a ribosome binding site are linked on the left flank and a double bidirectional terminator on the right flank of the recombinase.


Performance

Promotor lac promotor
RBS efficiency 1
Rci site specific recombinase BBa_K361000
terminator double bidirectional terminator

Here is our system leading to the generation of Rci site-specific recombinase (yellow in colour) Rci sys.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 588
    Illegal NgoMIV site found at 1083
  • 1000
    COMPATIBLE WITH RFC[1000]