Difference between revisions of "Part:BBa K496003"
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<partinfo>BBa_K496003 short</partinfo>
 
<partinfo>BBa_K496003 short</partinfo>
  
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leucine-rich repeat protein (SlrP)
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Leucine-rich repeat protein (SlrP)
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One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.
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One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins as GFP.
  
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See [[https://parts.igem.org/Part:BBa_K496004 BBa_K496004]]
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See [https://parts.igem.org/Part:BBa_K496004 BBa_K496004] for attempts to use this part to inject GFP to eukaryotic cell.
  
 
=References=
 
=References=
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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*Used to secrete tagged proteins through Type 3 Secretion Apparatus
  
 
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Revision as of 22:10, 27 October 2010

RBS + SlrP secretion tag, salmonella T3 sec. sys.

Leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins as GFP.

See BBa_K496004 for attempts to use this part to inject GFP to eukaryotic cell.

References

  1. Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]