Revision as of 22:08, 27 October 2010

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Characterization of BBa_K374006

Positive feedback antitermination on double reporter plasmid

The iGEM DTU team 2010 have submitted this part, after using it in the development of a genetic bistable switch.

Aim and strategy

The aim of the characterization was to asses if this part together with BBa_K374005 could have an antiterminating function that could be used as a regulatory mechanism.

We characterized the functionality of the part in the following construct.

caption

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we tested if:

If it was possible to trigger a positive feedback mechanism of N protein, by varying the promoter strength with the synthetic promoter library (SPL) and thus antitermination and constant expression of the down stream RFP.
If it was possible to trigger this positive feed back mechanism by inducing the N protein from a second plasmid insert with the pBAD promoter and the N protein.

Results

Conclusionc and Recommendations

User Reviews

UNIQd9306a9bbb6aff2d-partinfo-00000001-QINU

BBa_K374006 1 Not understood iGEM DTU 2010

We have characterized this part.

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UNIQd9306a9bbb6aff2d-partinfo-00000003-QINU

@@ Line 9: / Line 9: @@
 The iGEM DTU team 2010 have submitted this part, after using it in the development of a genetic bistable switch.
-=== Aim and strategy ===
+==== Aim and strategy ====
 The aim of the characterization was to asses if this part together with <partinfo>BBa_K374005 </partinfo> could have an antiterminating function that could be used as a regulatory mechanism.
 We characterized the functionality of the part in the following construct.
-[[Image:AntiT1.png|center|frame|200px|caption]]
+[[Image:AntiT1.png|center|frame|100px|caption]]
 XXXXXXXXXXXXXXXXplenation to the figure and the parts it consists of.