Difference between revisions of "Part:BBa K496003"

 
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<partinfo>BBa_K496003 short</partinfo>
 
<partinfo>BBa_K496003 short</partinfo>
  
 
leucine-rich repeat protein (SlrP)
 
leucine-rich repeat protein (SlrP)
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One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional restrictions of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.
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One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.
  
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Currently this part is in trials to see if it will secrete GFP. Chassis is E.coli with BAC vector fragment (SGSC4024  1464540-1562427) of salmonella SPI2.  
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See [[https://parts.igem.org/Part:BBa_K496004 BBa_K496004]]
  
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Please consult papers bellow about details
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=References=
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A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium
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# Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]
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Edward A. Miao and Samuel I. Miller
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2000
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Revision as of 22:07, 27 October 2010

RBS + SlrP secretion tag, salmonella T3 sec. sys.

leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.

See [BBa_K496004]

References

  1. Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]