Difference between revisions of "Part:BBa K342000:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
We wanted to only synthesize the promoter with the site of regulation by csgD. After analyzing the sequence, we decided to begin our promoter 20bp before the csgD box, and to finish it on the +1bp of transcription. It corresponds to 71 bp.<br> | We wanted to only synthesize the promoter with the site of regulation by csgD. After analyzing the sequence, we decided to begin our promoter 20bp before the csgD box, and to finish it on the +1bp of transcription. It corresponds to 71 bp.<br> | ||
| − | We added a non-CDS (non coding sequence) prefix and suffix to our sequence, to fit with iGEM standards. | + | We added a non-CDS (non coding sequence) prefix and suffix to our sequence, to fit with iGEM standards.<br> |
Need the part BBa_K342003 to be functional. | Need the part BBa_K342003 to be functional. | ||
Revision as of 21:54, 27 October 2010
Curli Promoter induced by temperature, shaking speed and osmolarity,
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to only synthesize the promoter with the site of regulation by csgD. After analyzing the sequence, we decided to begin our promoter 20bp before the csgD box, and to finish it on the +1bp of transcription. It corresponds to 71 bp.
We added a non-CDS (non coding sequence) prefix and suffix to our sequence, to fit with iGEM standards.
Need the part BBa_K342003 to be functional.