Difference between revisions of "Part:BBa K371024:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K371024 short</partinfo>
 
<partinfo>BBa_K371024 short</partinfo>
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===Design Notes===
 
===Design Notes===
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different codons of Gly and Ser was choosed in order to ignore the mismatch between forward sequence and reverse sequence when they are annealing.
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Different codons of Gly and Ser was choosed in order to ignore the mismatch between forward sequence and reverse sequence when they are annealing.Here is the oligonucleotides used to generate 10*GS.
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<html>
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<div style="background-color:#FFFACD";padding-left:5px;>
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<p>forward sequence:</p>
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<p>5'-GGTGGTAGCGGCAGCGGTAGCGGTAGCGGCAGC-3'</p>
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<p>reverse sequence:</p>
  
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<p>5'-CATGCTGCCGCTACCGCTACCGCTGCCGCTACC-3'</p>
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</div>
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</html>
  
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Then the product can directly be used to ligate with protein domain to make fusion protein according to BBF RFC 53.
  
 
===Source===
 
===Source===

Latest revision as of 21:51, 27 October 2010

10*GS linker(for RFC 53 protein fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different codons of Gly and Ser was choosed in order to ignore the mismatch between forward sequence and reverse sequence when they are annealing.Here is the oligonucleotides used to generate 10*GS.

forward sequence:

5'-GGTGGTAGCGGCAGCGGTAGCGGTAGCGGCAGC-3'

reverse sequence:

5'-CATGCTGCCGCTACCGCTACCGCTGCCGCTACC-3'

Then the product can directly be used to ligate with protein domain to make fusion protein according to BBF RFC 53.

Source

This part is not long, so it can be easily get by direct annealing two oligonucleotides.

References