Difference between revisions of "Part:BBa K354001:Design"

 
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===References===
 
===References===
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[1] Slamti L. & Lereclus D. (2002), A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. The European Molecular Biology Organisation Journal 21: 4550-4559.
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[2] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823.
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[3] Optimizer, http://genomes.urv.es/OPTIMIZER/, Last accessed September 29, 2010 [4] Marco Weinberg, PhD. Antiviral Gene Therapy Research Unit, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, 7 York Rd. Parktown 2193 SOUTH AFRICA, marc.weinberg@wits.ac.za.

Revision as of 21:27, 27 October 2010

PlcR-PapR - Gram+ve Quorum Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence recognised by the SecA pathway had to be included in the synthesised sequence so as to ensure that the fusion peptide is adequately exported fro the cell. A ribozyme binding site (RBS) was also included in the final sequence. Furthermore, the sequence was codon optimized for Lactobacillus gasseri.


Source

This sequence was adapted from the naturally-occurring fusion peptide found in the Bacillus cereus genome.

References

[1] Slamti L. & Lereclus D. (2002), A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. The European Molecular Biology Organisation Journal 21: 4550-4559.

[2] Pomerantsev A.P., Pomerantseva O.M. & Leppla S.H. (2004), A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependant Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence. Infection and Immunity 72:5814-5823.

[3] Optimizer, http://genomes.urv.es/OPTIMIZER/, Last accessed September 29, 2010 [4] Marco Weinberg, PhD. Antiviral Gene Therapy Research Unit, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, 7 York Rd. Parktown 2193 SOUTH AFRICA, marc.weinberg@wits.ac.za.