Difference between revisions of "Part:BBa K361001"
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In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
 
In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
  
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[http://137.189.51.115/igem/images/stories/msg.png   Message_system]
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[[Image:http://137.189.51.115/igem/images/stories/msg.png]]
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*Reference:
 
*Reference:

Revision as of 21:24, 27 October 2010

Message gene template

This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.

  • This message has 70 characters:

We must learn to live together as brothers or perish together as tools

  • In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

File:Http://137.189.51.115/igem/images/stories/msg.png


  • Reference:

Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
    Illegal BamHI site found at 427
    Illegal XhoI site found at 433
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]