Difference between revisions of "Part:BBa K313009"
| Line 2: | Line 2: | ||
<partinfo>BBa_K313009 short</partinfo> | <partinfo>BBa_K313009 short</partinfo> | ||
| − | This is a device with T7 promoter to detect the function of | + | This is a device with the T7 promoter to detect the function of the Cre recombinase. |
| − | If | + | |
| + | If Cre recombinase is expressed in the cell, the double-terminator will be excised irreversibly and GFP will be expressed by the T7 RNA polymerase. | ||
| + | |||
| + | You may be able to quantify the efficiency of the recombination by Cre by measuring the fluorescence intensity of GFP. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 21:18, 27 October 2010
cre recombinase assay device
This is a device with the T7 promoter to detect the function of the Cre recombinase.
If Cre recombinase is expressed in the cell, the double-terminator will be excised irreversibly and GFP will be expressed by the T7 RNA polymerase.
You may be able to quantify the efficiency of the recombination by Cre by measuring the fluorescence intensity of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 940