Difference between revisions of "Part:BBa K319041:Design"
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− | + | The nat gene was first isolated from Streptomyces noursei. [1] This gene provides the organism with resistance to the antibiotic nourseothricin. In 1999, Goldstein and McCusker produced a nat cassette based on the kanMX cassettes of plasmid pFA6 and showed that this new cassette confers resistance to the above mentioned drug in ''S. cerevisiae'' strain YAG44. We have BioBricked the complete cassette (including the upstream TEF1 promoter and the downstream TEF1 terminator) and have shown that it has 100% selectivity in ''S. cerevisiae'' YPH500 strain. | |
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===Source=== | ===Source=== |
Revision as of 21:09, 27 October 2010
NatMX cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 690
Illegal NgoMIV site found at 779
Illegal AgeI site found at 408 - 1000COMPATIBLE WITH RFC[1000]
Backgournd
The nat gene was first isolated from Streptomyces noursei. [1] This gene provides the organism with resistance to the antibiotic nourseothricin. In 1999, Goldstein and McCusker produced a nat cassette based on the kanMX cassettes of plasmid pFA6 and showed that this new cassette confers resistance to the above mentioned drug in S. cerevisiae strain YAG44. We have BioBricked the complete cassette (including the upstream TEF1 promoter and the downstream TEF1 terminator) and have shown that it has 100% selectivity in S. cerevisiae YPH500 strain.
Source
n