Difference between revisions of "Part:BBa K361001"

Revision as of 20:49, 27 October 2010

Message gene template

This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.

This message has 70 characters:

We must learn to live together as brothers or perish together as tools

In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

Reference:

Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 10
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 404
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 7
Illegal BamHI site found at 427
Illegal XhoI site found at 433
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 10
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 10
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K361001 short</partinfo>
 This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.
+*This message has 70 characters:
+We must learn to live together as brothers or perish together as tools
+*In a 4 bits system, the total sequence is 280 bp.
+In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
+*Reference:
+Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.
 <!-- Add more about the biology of this part here