Difference between revisions of "Part:BBa K424018"
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Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (rhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid but the natural rhlAB gene have illegal restriction sites that make it incompatible with the Assembly Standard 10. | Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (rhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid but the natural rhlAB gene have illegal restriction sites that make it incompatible with the Assembly Standard 10. | ||
How to used it? | How to used it? | ||
| − | We developed this standarized part to be inserted into ''E. coli'' for rhamnolipid production through the mutation of the key enzyme rhamnosyltranferase (rhlAB) to be compatible with Assembly Standard 10 . For this we do a multiple site mutagenesis | + | We developed this standarized part to be inserted into ''E. coli'' for rhamnolipid production through the mutation of the key enzyme rhamnosyltranferase (rhlAB) to be compatible with Assembly Standard 10 . For this we do to the natural rhlAB gene a multiple site mutagenesis. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:46, 27 October 2010
Rhamnosiltransferase BioBrick (Rh1AB_BB)
Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (rhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid but the natural rhlAB gene have illegal restriction sites that make it incompatible with the Assembly Standard 10. How to used it? We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the key enzyme rhamnosyltranferase (rhlAB) to be compatible with Assembly Standard 10 . For this we do to the natural rhlAB gene a multiple site mutagenesis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 169
Illegal BamHI site found at 729
Illegal XhoI site found at 905
Illegal XhoI site found at 2191 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1084
Illegal NgoMIV site found at 1805
Illegal NgoMIV site found at 1918 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 394
Illegal BsaI site found at 1434
Illegal BsaI.rc site found at 578