Difference between revisions of "Part:BBa K323005:Design"

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===Source===
 
===Source===
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Sequence of zinc finger PBSII was synthesised by GeneArt, whereas sequence of YFP is a commercially available.
  
 
===References===
 
===References===

Revision as of 20:29, 27 October 2010

CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence of zinc finger PBSII was synthesised by GeneArt, whereas sequence of YFP is a commercially available.

References

Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545

Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89