Difference between revisions of "Part:BBa K361000"
| Line 7: | Line 7: | ||
This is a Wide type Rci recombinase. | This is a Wide type Rci recombinase. | ||
| − | In the recombination activity study: | + | *In the recombination activity study: |
| − | Intramolecular inversion frequency = 0.062 | + | *Intramolecular inversion frequency = 0.062 |
| − | Intramolecular deletion frequency = 0 (recombinase inactive on this function) | + | *Intramolecular deletion frequency = 0 (recombinase inactive on this function) |
| − | Intermolecular recombination = 0 (recombinase inactive on this function) | + | *Intermolecular recombination = 0 (recombinase inactive on this function) |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The Rci site-specific recombinase is used for the bacteria to adapt the change in environment by rearrange their gene pattern to create a new species of bacteria that has selective advantage in that changed environment for survival. | + | *The Rci site-specific recombinase is used for the bacteria to adapt the change in environment by rearrange their gene pattern to create a new species of bacteria that has selective advantage in that changed environment for survival. |
| − | The Rci site-specific recombinase is now exploited to randomly rearrange the DNA sequence carrying data. It allows data encryption to be possible. | + | *The Rci site-specific recombinase is now exploited to randomly rearrange the DNA sequence carrying data. It allows data encryption to be possible. |
| − | + | *Reference: | |
| − | Reference: | + | |
Gyohda, A., Zhu, S., Furuya, N. & Komano, T. (2005). Asymmetry of Shufflon-specific Recombination Sites in Plasmid R65 Inhibits Recombination between Direct sfx Sequences. The Journal of Biological Chemistry, 281 (30), 20772-20779. | Gyohda, A., Zhu, S., Furuya, N. & Komano, T. (2005). Asymmetry of Shufflon-specific Recombination Sites in Plasmid R65 Inhibits Recombination between Direct sfx Sequences. The Journal of Biological Chemistry, 281 (30), 20772-20779. | ||
Revision as of 20:20, 27 October 2010
Rci site-specific recombinase
This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition.
After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
This is a Wide type Rci recombinase.
- In the recombination activity study:
- Intramolecular inversion frequency = 0.062
- Intramolecular deletion frequency = 0 (recombinase inactive on this function)
- Intermolecular recombination = 0 (recombinase inactive on this function)
Usage and Biology
- The Rci site-specific recombinase is used for the bacteria to adapt the change in environment by rearrange their gene pattern to create a new species of bacteria that has selective advantage in that changed environment for survival.
- The Rci site-specific recombinase is now exploited to randomly rearrange the DNA sequence carrying data. It allows data encryption to be possible.
- Reference:
Gyohda, A., Zhu, S., Furuya, N. & Komano, T. (2005). Asymmetry of Shufflon-specific Recombination Sites in Plasmid R65 Inhibits Recombination between Direct sfx Sequences. The Journal of Biological Chemistry, 281 (30), 20772-20779.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 362
Illegal NgoMIV site found at 857 - 1000COMPATIBLE WITH RFC[1000]