Difference between revisions of "Part:BBa K395600:Design"

Latest revision as of 19:50, 27 October 2010

Apple fragrance enzyme (MpAAT1) generator

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1666
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 205
Illegal AgeI site found at 1419
1000
COMPATIBLE WITH RFC[1000]

Design Notes

T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag

Source

The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).

References

Edwige J. F. Souleyre et al. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor, FEBS Journal, 272, 3132–3144 (2005)

Revision as of 19:50, 27 October 2010 (view source) Matsubara (Talk \| contribs) (→‎Source) ← Older edit		Latest revision as of 19:50, 27 October 2010 (view source) Matsubara (Talk \| contribs) (→‎References)
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	===References===		===References===
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		+	Edwige J. F. Souleyre et al. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor, FEBS Journal, 272, 3132–3144 (2005)