Difference between revisions of "Part:BBa K395600:Design"

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===Source===
 
===Source===
  
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apThe gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).
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The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).
  
 
===References===
 
===References===

Revision as of 19:50, 27 October 2010

Apple fragrance enzyme (MpAAT1) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
    Illegal BamHI site found at 1635
    Illegal XhoI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
    Illegal AgeI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag


Source

The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).

References