Difference between revisions of "Part:BBa K316024"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K316024 short</partinfo> | <partinfo>BBa_K316024 short</partinfo> | ||
| + | |||
This part is identical to <bbpart>BBa_K143023</bbpart> except for the dif site<bbpart>BBa_K3160</bbpart> integrated behind the 5' amye <bbpart>BBa_K143001</bbpart> integration sequence. | This part is identical to <bbpart>BBa_K143023</bbpart> except for the dif site<bbpart>BBa_K3160</bbpart> integrated behind the 5' amye <bbpart>BBa_K143001</bbpart> integration sequence. | ||
| Line 11: | Line 11: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | |||
| + | [[Image:VectorAMYE5d.PNG|center|800px]] | ||
| + | |||
<!-- --> | <!-- --> | ||
Revision as of 18:45, 27 October 2010
Chloraphenicol resistance gene with dif and 5amyE
This part is identical to BBa_K143023 except for the dif siteBBa_K3160 integrated behind the 5' amye BBa_K143001 integration sequence.
Chloramphenicol acetyltransferase (CAT) BBa_J31005 confers resistance to chloramphenicol, a common lab selection antibiotic. The 5' amyE BBa_K143001 integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence BBa_K143002 is also required.
This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce BBa_K143002.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 529
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]