Difference between revisions of "Part:BBa R0061:Experience"

Revision as of 18:37, 27 October 2010

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Applications of BBa_R0061

User Reviews

UNIQ3b5e96c1d12a3125-partinfo-00000000-QINU

No review score entered. iGEM Tokyo_Tech 2010
In order to characterize R0061, Plux repression promoter, we constructed K395101 combining R0061 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1 and used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control.

Overnight cultures of reporter strains grown at 37 °C containing appropriated antibiotics were diluted at least 1:100 and incubated at 37 °C as fresh cultures. After their OD₅₉₀ reached 0.6, added 3OC6HSL. After 3 hours of induction, fluorescence intensity was measured with flow cytometry.

After 3 hours of induction by 3OC6HSL, the expression of GFP with 3OC6HSL dropped to 1/3 comparing with the expression without 3OC6HSL.

We confirmed that this promoter works correctly. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep more information])

UNIQ3b5e96c1d12a3125-partinfo-00000002-QINU

iGEM Chiba 2010

we also cheracterized about R0061.

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 After 3 hours of induction by 3OC6HSL, the expression of GFP with 3OC6HSL dropped to 1/3 comparing with the expression without 3OC6HSL. <br><br>
 We confirmed that this promoter works correctly.
+(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep more information])
 <br>[[IMAGE:Tokyotech R0061 K395008 graph R0061.jpg|300px|none|left|thumb]]