Difference between revisions of "Part:BBa K382003"

 
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<partinfo>BBa_K382003 short</partinfo>
 
<partinfo>BBa_K382003 short</partinfo>
  
 
Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive promoter upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.
 
Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive promoter upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.
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We used this vector for integration of several gene constructs into ''Arabidopsis''.[http://2010.igem.org/Team:Harvard/results]
  
  

Revision as of 18:19, 27 October 2010

pORE Expression Series Vector with BioBrick MCS (Kan reistance)

Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive promoter upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.

We used this vector for integration of several gene constructs into Arabidopsis.[http://2010.igem.org/Team:Harvard/results]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5269
    Illegal XbaI site found at 5284
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5269
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
    Illegal NotI site found at 5275
    Illegal NotI site found at 5305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5269
    Illegal BglII site found at 5182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5269
    Illegal suffix found in sequence at 5298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5269
    Illegal XbaI site found at 5284
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 7160
    Illegal NgoMIV site found at 7795
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 7644
    Illegal SapI.rc site found at 7854