Difference between revisions of "Part:BBa K395101"
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This part is GFP reporter which is repressed by LuxR and 3OC6HSL, and the promoter is R0061. And we use this part to measure the activity of BBa_R0061. | This part is GFP reporter which is repressed by LuxR and 3OC6HSL, and the promoter is R0061. And we use this part to measure the activity of BBa_R0061. | ||
[[IMAGE:Tokyotech R0061 K395008 graph R0061.jpg|300px|left|thumb]] | [[IMAGE:Tokyotech R0061 K395008 graph R0061.jpg|300px|left|thumb]] | ||
| − | In order to measure the activity of R0061, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395101 into DH5α. We confirmed that R0061 is repressed by 3OC6HSL and LuxR as we expected. | + | In order to measure the activity of R0061, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395101 into DH5α. We confirmed that R0061 is repressed by 3OC6HSL and LuxR as we expected. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep more information]) |
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Latest revision as of 18:09, 27 October 2010
GFP reporter repressed by LuxR and 3OC6HSL (R0061:K121013)
This part is GFP reporter which is repressed by LuxR and 3OC6HSL, and the promoter is R0061. And we use this part to measure the activity of BBa_R0061.
In order to measure the activity of R0061, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395101 into DH5α. We confirmed that R0061 is repressed by 3OC6HSL and LuxR as we expected. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 64
Illegal BamHI site found at 53 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 756