Difference between revisions of "Part:BBa K395008"

Revision as of 18:04, 27 October 2010

Plux (rep) R0061middle

R0061 is the promoter repressed by LuxR in the presence of AHL. We designed this promoter by changing one base of the existing BioBrick (BBa_R0061).

Tokyotech R0061 K395008 graph K395008.jpg

In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1. In order to measure activity of R0061, we introduced K395105 and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep#R1, characterization of R0061 & K395008 (promoter repressed by LuxR/3OC6HSL) more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 We designed this promoter by changing one base of the existing BioBrick (BBa_R0061).
 [[IMAGE:Tokyotech R0061 K395008 graph K395008.jpg|400px|left|thumb]]
-In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1. In order to measure activity of R0061, we introduced K395105 and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep#R1, characterization of R0061 & K395008 (promoter repressed by LuxR/3OC6HSL) more information])
+In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1. In order to measure activity of R0061, we introduced K395105 and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep#R1, characterization of R0061 & K395008 (promoter repressed by LuxR/3OC6HSL)  more information])