Difference between revisions of "Part:BBa K305001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | codon optimized for B. subtilis | + | The RBS sequence used in this part can be found in several B. subtilis genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the B. subtilis wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images). |
| − | + | [[Image:C codon opt Groningen.jpg]][[C codon opt Groningen.jpg]] | |
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 17:48, 27 October 2010
Hydrophobic protein chaplin C (chpC)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 349
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 533
Design Notes
The RBS sequence used in this part can be found in several B. subtilis genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the B. subtilis wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images).
C codon opt Groningen.jpg
Source
Ordered as construct from Mr. Gene after genomic sequence had been identified.
References
Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726