Difference between revisions of "Part:BBa K395105"

 
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<partinfo>BBa_K395105 short</partinfo>
 
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This part is GFP reporter which is represed by LuxR and 3OC6HSL, and the promoter is K395008. And we use this part to measure the activity of BBa_K395008.
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[[IMAGE:Tokyotech R0061 K395008 graph K395008.jpg|400px|left|thumb]]
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In order to measure activity of R0061, we constructed this BioBrick combining LuxR repression promoter (K395008) and gfp reporter (K121013)on pSB6A1.  
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When we measured the fluorescence, we introduced this BioBrick and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:45, 27 October 2010

GFP reporter repressed by LuxR and 3OC6HSL (K395008:K121013)


Tokyotech R0061 K395008 graph K395008.jpg

In order to measure activity of R0061, we constructed this BioBrick combining LuxR repression promoter (K395008) and gfp reporter (K121013)on pSB6A1.


When we measured the fluorescence, we introduced this BioBrick and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal BamHI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 756