Difference between revisions of "Part:BBa K313009"
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This is a device with T7 promoter to detect the function of cre recombinase.
 
This is a device with T7 promoter to detect the function of cre recombinase.
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If cre recombinase expresses in the cell, the double-terminator will be cut out irreversibly and gfp will be expressed by T7 RNA polymerase.
+
If cre recombinase is expressed in the cell, the double-terminator will be cut out irreversibly and gfp will be expressed by T7 RNA polymerase.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:40, 27 October 2010

cre recombinase assay device

This is a device with T7 promoter to detect the function of cre recombinase. If cre recombinase is expressed in the cell, the double-terminator will be cut out irreversibly and gfp will be expressed by T7 RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 940