Difference between revisions of "Part:BBa K389014"

(Usage and Biology)
(Usage and Biology)
 
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===Usage and Biology===
 
This Device can be used for characterization and screening of mutated VirA receptors.
 
  
For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389016</partinfo>
 
  
  

Latest revision as of 17:04, 27 October 2010

VirA/G screening system test

This BioBrick is made to test our virA screening system and the strategy for directed evolution, respectively. It contains an unmutated virA under the control of a constitutive promoter and the screening device BBa_K389011.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768