Difference between revisions of "Part:BBa K389014"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389014 short</partinfo>
 
<partinfo>BBa_K389014 short</partinfo>
Line 5: Line 4:
 
This BioBrick is made to test our ''virA'' screening system and the strategy for directed evolution, respectively. It contains an unmutated ''virA'' under the control of a constitutive promoter and the screening device <partinfo>K389011</partinfo>.  
 
This BioBrick is made to test our ''virA'' screening system and the strategy for directed evolution, respectively. It contains an unmutated ''virA'' under the control of a constitutive promoter and the screening device <partinfo>K389011</partinfo>.  
  
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This Device can be used for characterization and screening of mutated VirA receptors.
 +
 +
For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389015</partinfo>
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 17:03, 27 October 2010

VirA/G screening system test

This BioBrick is made to test our virA screening system and the strategy for directed evolution, respectively. It contains an unmutated virA under the control of a constitutive promoter and the screening device BBa_K389011.


Usage and Biology

This Device can be used for characterization and screening of mutated VirA receptors.

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389015


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768