Difference between revisions of "Part:BBa K389012"
Line 8: Line 8:
 
For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find thh complete working system here: <partinfo>K389015</partinfo>
 
For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find thh complete working system here: <partinfo>K389015</partinfo>
  
−
This Device can be used for characterization and scrrening of mutated VirA receptors.
+
This Device can be used for characterization and screening of mutated VirA receptors.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:49, 27 October 2010

VirA reporter system luc

With this system it is possible to measure the activity of a VirA receptor and the vir promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10[luc2] plasmid.


Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find thh complete working system here: BBa_K389015

This Device can be used for characterization and screening of mutated VirA receptors. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2539
    Illegal NgoMIV site found at 2560
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2445