Difference between revisions of "Part:BBa K320005"

Revision as of 16:17, 27 October 2010

Constitutive expression of CFP

This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).

This BioBrick was principally built to test the constitutive promoter we syntetise (BBa_K320002).

We only manage to characterize it in E.Coli (see the experiement page). Further on, we will characterize it in Asaia to continue building gear for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 62
Illegal NheI site found at 146
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
-This BioBrick was principally build to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>).
+This BioBrick was principally built to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>).
-We only manage to caracterize it in E.Coli (see the experiement page). Further we will caractreize it in Asaia to continue building gear for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia''].
+We only manage to characterize it in E.Coli (see the experiement page). Further on, we will characterize it in Asaia to continue building gear for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia''].
 <!-- Add more about the biology of this part here