Difference between revisions of "Part:BBa K346096"

Revision as of 16:14, 27 October 2010

PmerT promoter-GFP(E0840)

This part was designed to characterize PmerT. When this plasmid was transformed to the bacteria expressing its correspondent activator MerR, the strain would be induced by Hg(II) to express GFP. The relationship between GFP intensity and the Hg(II)'s concentration helped us learn the character of PmerT.

We constructed this part to calculate the expression level of GFP under the wild type PmerT. The plot is done by using different concentration of Hg to induce the promoter and to calculate the GFP intensity. In detail, when the concentration of Hg(II) in the cytosol is different, the active form of MerR dimer will increase and the possibility that it will active PmerT promoter will increase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 730

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K346096 short</partinfo>
-PmerT promoter-GFP(E0840)
+This part was designed to characterize PmerT. When this plasmid was transformed to the bacteria expressing its correspondent activator MerR, the strain would be induced by Hg(II) to express GFP. The relationship between GFP intensity and the Hg(II)'s concentration helped us learn the character of PmerT.
+[[Image:merT-E0840.jpg|500px|center]]
+We constructed this part to calculate the expression level of GFP under the wild type PmerT. The plot is done by using different concentration of Hg to induce the promoter and to calculate the GFP intensity. In detail, when the concentration of Hg(II) in the cytosol is different, the active form of MerR dimer will increase and the possibility that it will active PmerT promoter will increase.
+[[Image:merT-wt.jpg|center]]