Difference between revisions of "Part:BBa K320004:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
We ligate BioBricks <partinfo>BBa_K320000</partinfo> and <partinfo>BBa_P1003</partinfo>  
 
We ligate BioBricks <partinfo>BBa_K320000</partinfo> and <partinfo>BBa_P1003</partinfo>  
  
−
The first BioBricks (<partinfo>BBa_K320000</partinfo>) was intial in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzyme SpeI and PstI.  
+
The first BioBrick (<partinfo>BBa_K320000</partinfo>) was intial in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzyme SpeI and PstI.  
  
−
The second part (<partinfo>BBa_p1003</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. This one we digested with Xba and PstI.
+
The second BioBrick (<partinfo>BBa_p1003</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. This one we digested with Xba and PstI.
  
 
===Source===
 
===Source===

Revision as of 16:04, 27 October 2010

Origin for Asaia and resistance to Kanamycin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


Design Notes

We ligate BioBricks BBa_K320000 and BBa_P1003

The first BioBrick (BBa_K320000) was intial in the pSB1C3 backbone vector. We opened it with the restriction enzyme SpeI and PstI.

The second BioBrick (BBa_P1003) was also stored in the pSB1C3 backbone vector. This one we digested with Xba and PstI.

Source

The BioBricks assembled from BBa_K320000 and BBa_P1003.

References