Difference between revisions of "Part:BBa K398029"
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This part was characterized in the plasmid pSB1A2. You can see our original plasmid map below. | This part was characterized in the plasmid pSB1A2. You can see our original plasmid map below. | ||
| − | [[Image:TUDelftALDH_map.jpg|600px|thumb| | + | [[Image:TUDelftALDH_map.jpg|600px|thumb|general|Comparison of ALDH activities in the different cell extracts tested in our study.]] |
Revision as of 16:00, 27 October 2010
ALDH generator (low expression)
ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through β-oxidation
The [http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation characterization of BBa_K398029] has been described on the TU Delft iGEM Team 2010 wiki.
Usage and Biology
Aldehyde dehydrogenase from the thermophile Geobacillus thermoleovorans B23. It functions as an octamer, requiring NAD+ as coenzyme. The optimum condition for activity lies at temperatures between 50 and 55 degrees celsius and and a pH of 10. This part also works fine at 37º C.
This part was characterized in the plasmid pSB1A2. You can see our original plasmid map below.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
- 1000COMPATIBLE WITH RFC[1000]
[1] Kato, T., et al., Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23. Extremophiles, 2010. 14(1): p. 33-39.