Difference between revisions of "Part:BBa K320006:Design"
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We ligate two BioBricks quoted below. | We ligate two BioBricks quoted below. | ||
| − | + | The first BioBricks (<partinfo>BBa_K320001</partinfo>) was intial in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzyme SpeI and PstI. | |
| + | |||
| + | The second part (<partinfo>BBa-K320002</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. This one we digested with Xba and PstI. | ||
===Source=== | ===Source=== | ||
Revision as of 15:54, 27 October 2010
Constitutive expression of Immunotoxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 883
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ligate two BioBricks quoted below.
The first BioBricks (BBa_K320001) was intial in the pSB1C3 backbone vector. We opened it with the restriction enzyme SpeI and PstI.
The second part (BBa_K320002) was also stored in the pSB1C3 backbone vector. This one we digested with Xba and PstI.
Source
The Two BioBricks we used are BBa_K320001 and BBa-K320002.