Difference between revisions of "Part:BBa K352004"

Latest revision as of 15:32, 27 October 2010

G2C Mutated pCooF from Rhodospirillum rubrum

This part is the mutated version of pCooF promoter. The mutation involves conversion guanine to cytosine at second base. The binding strength of pCooF to CooA is expected to diminish.

Rhodospirillum rubrum is able to use CO as an carbon source with its CO-sensing protein called CooA. CooA activates the transcription of genes encoding the CO-oxidation (coo) regulon which produces the proteins needed for the utilization of CO. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.

The two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ, is responsible for the CO-dependent anaerobic growth of Rhodospirillum rubrum and enable the bacteria to oxidize CO . The coo regulon has three main products which are listed as O2-sensitive CO dehydrogenase (CooS), CooS-associated Fe-S protein (CooF), CO-tolerant hydrogenase (CooH). CooA is responsible for the expression of the genes of these proteins and activates by sensing CO in anaerobic environments.

Because of the lack of active CooA, CO-independent basal level transcription of PcooF is not detectable.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is the mutated version of pCooF promoter. The mutation involves conversion guanine to cytosine at second base. The binding strength of pCooF to CooA is expected to diminish.
-CooA is a CO-sensing protein that activates the transcription of genes encoding the CO-oxidation (coo) regulon, whose polypeptide products are required for utilizing CO as an energy source in Rhodospirillum rubrum. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.
+Rhodospirillum rubrum is able to use CO as an carbon source with its CO-sensing protein called CooA. CooA activates the transcription of genes encoding the CO-oxidation (coo) regulon which produces the proteins needed for the utilization of CO. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.
-The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
+The two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ, is responsible for the CO-dependent anaerobic growth of Rhodospirillum rubrum and enable the bacteria to oxidize CO . The coo regulon has three main products which are listed as O2-sensitive CO dehydrogenase (CooS), CooS-associated Fe-S protein (CooF), CO-tolerant hydrogenase (CooH). CooA is responsible for the expression of the genes of these proteins and activates by sensing CO in anaerobic environments.
-CO-independent basal level transcription of PcooF is not detectable due to the absence of active CooA.
+Because of the lack of active CooA, CO-independent basal level transcription of PcooF is not detectable.
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