Difference between revisions of "Part:BBa K274003:Experience"
(→User Reviews) |
(→User Reviews) |
||
Line 7: | Line 7: | ||
===User Reviews=== | ===User Reviews=== | ||
− | E.coli turned dark green after transformation of this plasmid | + | E. coli turned dark green after transformation of this plasmid. This is unexpected as there is no promoter upstream of the operon. |
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
Line 15: | Line 15: | ||
<I>UCL_London, Xiang Chen</I> | <I>UCL_London, Xiang Chen</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
− | |||
− | |||
Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below. | Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below. | ||
+ | |||
+ | This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts. | ||
+ | |||
[[Image:K274003 Diagnostic Gel .jpg|center|300px]] | [[Image:K274003 Diagnostic Gel .jpg|center|300px]] |
Revision as of 15:27, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K274003
User Reviews
E. coli turned dark green after transformation of this plasmid. This is unexpected as there is no promoter upstream of the operon.
BBa_K274003 Review No.2 UCL_London, Xiang Chen |
Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below. This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.
|
UNIQdb6f7a3cc5a58688-partinfo-00000000-QINU UNIQdb6f7a3cc5a58688-partinfo-00000001-QINU