Difference between revisions of "Part:BBa K320004:Experience"

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===Applications of BBa_K320004===
 
===Applications of BBa_K320004===
  
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We transformed E.Coli and Asaia with this biobrick in the pSBC3 backbone plasmid vector. We plated those cultur  respectively on Lb+Agar+Kanamycin and Gly+Agar+Kanamycin.  
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This BioBricks has two main fonction, permit the bacteria to resist to the Antibiotic Kanamycin and provide an origin of replication working for E.Coli and Asaia.  
  
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We observe that on both plates we obtain colonnies and that in negativ controls (E.Coli and Asaia without BBaK320004 in pSBC3 backbone vector) we obtain non colonnies.
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To provide the experiment we put the BioBrick in the pSBC3 vector. This vector provide a resistance to Chloramphenicole and an origin of replication which works only for E.Coli.
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We transfected E.Coli and Asaia with the plasmid.
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The transfect E.Coli were grown on a plat of LB with Agar an containing Kanamycin with a concentration of . This experiment permit us to confirm the providing of the resitance to Kanamycin to teh bacterias.
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The transfect Asaia were grown on a plat of Gly with Agar an containing Kanamycin with a concentration of ...(according to our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia "characterisation of Asaia"]. This experiment permit us to confirm the providing of the origin of replication.
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For each strand we made a negativ control with E.Coli or Asaia without plamsid.
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We observe that on both plates with transfected bacteria we obtain colonnies and that in negativ controls we obtain non colonnies.
  
 
Put Picture of the plates
 
Put Picture of the plates
  
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In conclusion we assume that our resitance to Kanamycin works and that the origin in replication also works in Asaia.
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In conclusion we assume that our resitance to Kanamycin works and that the origin in replication also works for Asaia.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 15:05, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K320004

This BioBricks has two main fonction, permit the bacteria to resist to the Antibiotic Kanamycin and provide an origin of replication working for E.Coli and Asaia.

To provide the experiment we put the BioBrick in the pSBC3 vector. This vector provide a resistance to Chloramphenicole and an origin of replication which works only for E.Coli.

We transfected E.Coli and Asaia with the plasmid.

The transfect E.Coli were grown on a plat of LB with Agar an containing Kanamycin with a concentration of . This experiment permit us to confirm the providing of the resitance to Kanamycin to teh bacterias.

The transfect Asaia were grown on a plat of Gly with Agar an containing Kanamycin with a concentration of ...(according to our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia "characterisation of Asaia"]. This experiment permit us to confirm the providing of the origin of replication.

For each strand we made a negativ control with E.Coli or Asaia without plamsid.

We observe that on both plates with transfected bacteria we obtain colonnies and that in negativ controls we obtain non colonnies.

Put Picture of the plates

In conclusion we assume that our resitance to Kanamycin works and that the origin in replication also works for Asaia.

User Reviews

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