Difference between revisions of "Part:BBa K346090:Design"

 
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<partinfo>BBa_K346090 short</partinfo>
 
<partinfo>BBa_K346090 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100)
 
  
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We find that BBa_K274100 represented a significant leakage expression when exploited as a reporter gene and even bacteria bearing BBa_K274100 only could also represent significant color change, compared with the blank. We speculate that it’s because of a putative promoter upstream, resulting in the leaky expression of CrtEBI. In order to verify the speculation, we further characterize this biobrick. This biobrick was suffixed to the constitutive promoter BBa_J23103.
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We also suffixed BBa_K274100 to the terminator BBa_B0015. Terminator upstream was expected to reduce the basal level of lycopene production, thus to verify our speculation(Fig.1).
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[[Image:crt-ter.jpg]]
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'''Fig.1. Biobricks we constructed to characterize biobrick BBa_K274100.''' The left construct denotes Constitutive promoter BBa_J23103-CrtEBI and on the right is an interesting biobrick—Terminator BBa_B0015-CrtEBI, namely a terminator was prefixed to CrtEBI, expected to reduce the leaky expression.
  
  
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===Source===
 
===Source===
  
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Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100)
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BBa_J23103 and CrtEBI(BBa_K274100) are both from parts-registry.
  
  
 
===References===
 
===References===

Revision as of 15:02, 27 October 2010

Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2017
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1553
    Illegal NgoMIV site found at 1683
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We find that BBa_K274100 represented a significant leakage expression when exploited as a reporter gene and even bacteria bearing BBa_K274100 only could also represent significant color change, compared with the blank. We speculate that it’s because of a putative promoter upstream, resulting in the leaky expression of CrtEBI. In order to verify the speculation, we further characterize this biobrick. This biobrick was suffixed to the constitutive promoter BBa_J23103.

We also suffixed BBa_K274100 to the terminator BBa_B0015. Terminator upstream was expected to reduce the basal level of lycopene production, thus to verify our speculation(Fig.1).


Crt-ter.jpg

Fig.1. Biobricks we constructed to characterize biobrick BBa_K274100. The left construct denotes Constitutive promoter BBa_J23103-CrtEBI and on the right is an interesting biobrick—Terminator BBa_B0015-CrtEBI, namely a terminator was prefixed to CrtEBI, expected to reduce the leaky expression.


Source

BBa_J23103 and CrtEBI(BBa_K274100) are both from parts-registry.


References