Difference between revisions of "Part:BBa K310007:Design"

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===References===
 
===References===
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This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]

Latest revision as of 14:47, 27 October 2010

ArsR repressor of the Ars Operon in E. coli K-12 MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Design Notes

Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.

Source

E. coli K-12 MG1655 genome

References

This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]