Difference between revisions of "Part:BBa K320005:Experience"
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===Applications of BBa_K320005=== | ===Applications of BBa_K320005=== | ||
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We used this BioBrick to characterize a strong promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br /> | We used this BioBrick to characterize a strong promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br /> | ||
Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | ||
Revision as of 14:47, 27 October 2010
Applications of BBa_K320005
We used this BioBrick to characterize a strong promoter we want to use in Asaia BBa_K320002. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.
Both measurements were done simultaneously in the E. coli strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.
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