Difference between revisions of "Part:BBa K320006:Experience"
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== The Immunotoxin is expressed and appears in the supernatant== | == The Immunotoxin is expressed and appears in the supernatant== |
Revision as of 14:45, 27 October 2010
Applications of BBa_K320006
The Immunotoxin is expressed and appears in the supernatant
We tested expression of the immunotoxin in E. coli (see [http://2010.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Project/Materials_Methods Materials and Methods] for details). In a western blot analysis of whole cell lysates we were able to see bands corresponding to full length immunotoxin and possibly degraded fragments of the protein (see figure). The immunotoxin contains a PelB sequence that targets it for secretion into the periplasm. We concentrated the supernatant of both the immunotoxin and a control culture by running it through a filtering device with a 5 kDa cut-off. Running a western blot with these samples (see figure) we could show that the immunotoxin was found in the supernatant as expected.
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