Difference between revisions of "Part:BBa K323080"
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| − | iGEM 2010 team Slovenia used this part along with 4 others [[https://parts.igem.org/Part:BBa_K323005 BBa_K323005], [https://parts.igem.org/Part:BBa_K323021 BBa_K323021], [https://parts.igem.org/Part:BBa_K323029 BBa_K323029], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323039 BBa_323039]] to exhibit FRET reconstitution on a predefined DNA scaffold in vivo by transfecting mammalian HEK293 cell line with these 5 plasmids. In particular this BioBrick is part of a FRET subsystem leading to split mCitrine reassembly. It consists of a C terminal fragment of mCitrine, a linker sequence and Zif268 zinc finger DNA binding domain. Reconstitution of a fluorescent protein was observed under confocal microscope. mCitrine was excited with 514nm Argon laser and emmision was detected in 520 to 580 nm window. Combining this 2-part subsystem with the other one, leading to mCerulean reassembly [[https://parts.igem.org/Part:BBa_K323021 BBa_K323021], [https://parts.igem.org/Part:BBa_K323029 BBa_K323029]], FRET on a DNA program can be observed. | + | iGEM 2010 team Slovenia used this part along with 4 others [[https://parts.igem.org/Part:BBa_K323005 BBa_K323005], [https://parts.igem.org/Part:BBa_K323021 BBa_K323021], [https://parts.igem.org/Part:BBa_K323029 BBa_K323029], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323039 BBa_323039]] to exhibit FRET reconstitution on a predefined DNA scaffold ''in vivo'' by transfecting mammalian HEK293 cell line with these 5 plasmids. In particular this BioBrick is part of a FRET subsystem leading to split mCitrine reassembly. It consists of a C terminal fragment of mCitrine, a linker sequence and Zif268 zinc finger DNA binding domain. Reconstitution of a fluorescent protein was observed under confocal microscope. mCitrine was excited with 514nm Argon laser and emmision was detected in 520 to 580 nm window. Combining this 2-part subsystem with the other one, leading to mCerulean reassembly [[https://parts.igem.org/Part:BBa_K323021 BBa_K323021], [https://parts.igem.org/Part:BBa_K323029 BBa_K323029]], FRET on a DNA program can be observed. |
Latest revision as of 14:19, 27 October 2010
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system
iGEM 2010 team Slovenia used this part along with 4 others [BBa_K323005, BBa_K323021, BBa_K323029, BBa_323039] to exhibit FRET reconstitution on a predefined DNA scaffold in vivo by transfecting mammalian HEK293 cell line with these 5 plasmids. In particular this BioBrick is part of a FRET subsystem leading to split mCitrine reassembly. It consists of a C terminal fragment of mCitrine, a linker sequence and Zif268 zinc finger DNA binding domain. Reconstitution of a fluorescent protein was observed under confocal microscope. mCitrine was excited with 514nm Argon laser and emmision was detected in 520 to 580 nm window. Combining this 2-part subsystem with the other one, leading to mCerulean reassembly [BBa_K323021, BBa_K323029], FRET on a DNA program can be observed.
Click Experience to find out more!
Check out more at [http://2010.igem.org/Team:Slovenia Team Slovenia]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]